Dr.
Amy Milsted
University
of Akron
Professor /
Department of Biology /
Akron, Ohio 44325-3908
Phone: (330) 972-7976 / Office: ASEC 279
/ Email: milsted@uakron.edu
In my laboratory we are currently studying gene expression and regulation in rats, in cell cultures derived from them and in other cultured mammalian cell models. My interests include the renin-angiotensin system and its role in hypertension, Y chromosome genes and evolution, and Sry transcription factor function. I am especially interested in determining what regulates Sry expression and what Sry itself regulates. This is part of a larger project to determine whether Sry is the Y chromosome gene responsible for gender differences in hypertension. My focus is on Sry transcripts and proteins. We are exploring post-translational modification of Sry as an additional level of regulation of function of this transcription factor. Current projects are listed below:
The roles of Sry genes in rat development. Discovering what else Sry does in addition to testis determination and at least one copy affecting blood pressure – current NIH funding
Sry protein post-translational modification – developing as a new project
Application of prokaryotic and eukaryotic cell cultures systems for testing biologically relevant properties of synthetic compounds – ongoing, good point for interaction with other disciplines in Integrated Biosciences
Hypertension and the role of genomic imprinting in female rats - this research was supported by the American Heart Association and NIH.
Gender differences in development and maintenance of hypertension
EDUCATION
Ph. D. The City University of New York. Cell biology
B. Sc. in Edu. Ohio State University. Secondary education: chemistry
PROFESSIONAL EXPERIENCE
2000-present: Professor, Dept. of Biology The University of Akron, Akron, OH
1993-2000: Associate Professor, Dept. of Biology, The University of Akron, Akron, OH
1995-present: Graduate Faculty, School of Biomedical Sciences, Kent State University, Kent, OH
Previous appointments at:
1- Cleveland Clinic Foundation, Dept. of Brain and Vascular Research/Neurosciences, Asst. Staff
2- Veterans Administration Medical Center, Research Chemist
3- Case Western Reserve University, Dept. of Medicine, Research Associate,
4- Carnegie-Mellon University, Pittsburgh, PA, Muscular Dystrophy Association Postdoctoral Fellow
AWARDS
• Central Ohio American Heart Association, 2004 Women's Heart Health Initiative Research Grant
Recipient
• Fellow, American Heart Association, Council for High Blood Pressure, elected 2001
• Visiting Professor Fellowship Award, Education Ministry of Brazil, 1993, 1995
COURSES TAUGHT
Molecular Biology, Cell and Molecular Biology, Basic DNA Techniques, Principles of Microbiology, Molecular Endocrinology
REPRESENTATIVE PUBLICATIONS
Milsted A, Marcelo MC, Turner ME, Ely .L: Female WKY and SHR/y rats have the same genotype but different patterns of expression of renin and angiotensinogen. J. Hypertens. 16:823-828, 1998.
Seachrist D, Dunphy G, Daneshvar H, Caplea A, Milsted A, Ely D: Testosterone increases blood pressure and cardiovascular and renal pathology in SHR. Blood Pressure 9:1-12, 2000.
Ely D, Turner M, Milsted A: Review of the Y chromosome and hypertension. Braz. J. Med. Biol. Res. 33:679-691, 2000.
Neves LAA, Santos RAS, Khosla MC, Milsted A: Angiotensin-(1-7) regulates the levels of Angiotensin II receptor subtype AT1 mRNA differentially in a strain-specific fashion. Regulatory Peptides 95:99-107, 2000.
Passerini AG, Milsted A, Rittgers SE: Shear stress magnitude and directionality modulate growth factor gene expression in preconditioned vascular endothelial cells. Journal of Vascular Surgery 37:182-190, 2003.
Turner M, Jenkins C, Milsted A, Ely D.: Chapter 1: Sex Chromosomes in "Principles of Sex Based Differences in Physiology", Advances in Molecular and Cell Biology, Vol. 34, pages 1-14, 2003.
Melaiye A, Simons RS, Milsted A, Pingitore F, Wesdemiotis C , Tessier CA, Youngs WJ: Formation of Water Soluble Pincer Silver (I) Carbene Complexes, A Novel Antimicrobial Agent. J. Med. Chem. 47:973-977, 2004.
Milsted A, Serova L, Sabban EL, Dunphy G, Turner ME, Ely DE: Regulation of tyrosine hydroxylase gene transcription by Sry. Neuroscience Letters. 369:203-207, 2004.
Kascatan-Nebioglu A, Melaiye A, Hindi K, Durmus S, Panzer MJ, Hogue LA, Mallett RJ, Hovis CE, Coughenour M, Crosby SD, Milsted A, Ely DL, Tessier CA, Cannon CL, Youngs WJ. Synthesis from caffeine of a mixed N-heterocyclic carbene-silver acetate complex active against resistant respiratory pathogens. J. Med. Chem. 49:6811-6818, 2006.
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