Dr. Richard Londraville
I'm a fish guy-I study how fish do what they do. I'm also a fat guy-I'm interested in what cells and organisms do with fat. Finally, I'm a temperature guy-I spend time thinking about how temperature affects cell function. Together, these things mean that I study the cell physiology of fish with different body temperatures, and different fat metabolisms. In the past I've worked on very cold fish (Antarctic fish) and fish that are cold seasonally (like striped bass). I've also worked on some fish that keep parts of their bodies warm (blue marlin). Where does fat come into the picture? Cold fish tend to accumulate fat. Why? How? That is some of the focus of our current research. We study proteins that may help the cell to burn fat, move it around, get rid of it or deposit it. Specifically, we are working on leptin, and enzymes that burn fat. Current directions include characterizing how leptin works in zebrafish and carp.
Currently I am working with 3 doctoral students in the new Integrated Bioscience PhD program. Donnie Copeland is working on how leptin affects carp's physiology in their environment. He is particularly interested in response to stress and cold. Hope Ball is working with myself, Joel Duff, and Hans Thewissen at NEOUCOM. Her project is investigating the biology of leptin in Bowhead whales. Mark Dalman will join the lab this Fall, and he is going to be working on leptin physiology in zebrafish.
Common techniques we use in the lab include proteomics, quantitative PCR, immunoblotting, microinjection of morpholino oligonucleotides, etc. (the common molecular bag of tricks).
1994 Ph.D. University of Maine, Orono
Londraville, R.L. and P.H. Niewiarowski. 2009. Leptin signaling systems in reptiles and amphibians. In: Leptin in Non-Mammalian Vertebrates. Ed. by M. Paolucci. Published by Research Signpost, Kerala, India. In press.
Heflin, B., Young, L., and R. Londraville. 2009. Short-term cycling of skin colouration in the blackspotted rockskipper Entomacrodus striatus. J. Fish Biol. 74: 1635-1641.
Jury, D.R., Kaveti, S., Duan, Z-H, Willard, B., Kinter, M. and R. Londraville. 2008. Effects of calorie restriction on the zebrafish proteome. Comp. Biochem. Physiol. D. 3:275-282.
Liu, Q., R.L. Londraville, J.A. Marrs, A.L. Wilson, T. Mbimba, T. Murakami, F. Kubota, W. Zheng, and D.G. Fatkins. 2008. Cadherin-6 function in zebrafish retinal development. Develop. Neurobiol. 68(8):1107-1122.
Liu, B., R.J. Duff, R.L. Londraville, J.A. Marrs and Q. Liu. 2007. Cloning and expression analysis of cadherin 7 in the central nervous system of the embryonic zebrafish. Gene Express. Patterns 7 (1-2): 15-22
Spanovich, S., P.H. Niewiarowski, and R. L. Londraville. 2006. Seasonal effects on circulating leptin in the lizard Sceloporus undulatus from two populations. Comp. Biochem. Physiol. 143(4): 507-513.
Crockett, E.L. and R. L. Londraville. 2005. Temperature. pp. 231-269. In: The Physiology of Fishes 3rd Edition. Ed. by D.H. Evans abd J.B. Crc Press, Boca Raton, FL.
Liu Q, and R. L. Londraville. 2003. Using adult zebrafish visual system to study cadherin-2 expression during central nervous system regeneration. Methods Cell Sci. 00:1-8.
Liu Q., R.L. Londraville, E. Azodi, S.G. Babb, C. Chiappini, J.A. Marrs, and P. A. Raymond. 2002. Up-Regulation of Cadherin-2 and Cadherin-4 in Regenerating Visual Structures of Adult Zebrafish. Exp. Neurology 177:396-406.
Tschantz, D.R., E.L. Crockett, P.H. Niewiarowski, and R.L. Londraville. 2002. Cold acclimation strategy is highly variable among the sunfishes (Centrarchidae) Physiol. Biochem. Zool. 75:544-556.
Johnson, R.M.,T.M. Johnson, and R.L. Londraville. 2000. Evidence for leptin expression in fishes. J. Exp. Zool. 286(7):718-724